AIR-seq tracks RNA synthesis and decay using standard RNA-seq libraries
lpachter · x · 2026-07-29
AIR-seq measures RNA synthesis and decay without chemical conversion
Steady-state RNA-seq only shows how much RNA is present, not the synthesis and decay rates that shape gene expression. This paper introduces analog intrinsic recoding sequencing (AIR-seq), which repurposes N4-hydroxycytidine (NHC) to mark newly synthesized RNA through C-to-T and T-to-C mismatches in standard RNA-seq libraries.
Key points:
- Avoids the chemical conversion and enrichment steps used in many RNA metabolic labeling methods.
- Works for both bulk and single-cell RNA dynamics.
- Improves RNA velocity inference.
- Reveals previously hidden regulation in gene expression dynamics.
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