New Genetics paper separates cis vs. trans contributions to gene expression differences

lpachter · x · 2026-09-16

Lior Pachter highlights a new Genetics paper by Ingileif Hallgrímsdóttir and Maria Carilli that quantifies cis vs. trans regulatory contributions to gene expression differences using homozygous strains and their hybrid crosses.

A follow-up analysis by Ryan Weber and the Calvi group applied the method to massive IGVF mouse sn-RNA-seq data (C57BL/6 crossed with 7 other strains), revealing a 'trans lacuna' in human genetics: without crosses, untangling regulation modalities remains a major challenge. Tools are open-sourced as XgeneR (R) and XgenePy (Python).

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